Stay One Step Ahead in HPV Diagnostics
Human Papillomavirus (HPV) is a globally prevalent virus known to cause various types of cancer. Early diagnosis of HPV infections is crucial for prevention and treatment. The MultiPap+ HPV Genotyping Kit provides a reliable and sensitive solution for the detection of rare HPV types, including HPV31, HPV33, HPV35, HPV39, HPV51, HPV52, HPV56, HPV58, HPV59, HPV68, and HPV69.
This kit is developed to qualitatively detect 11 rare high-risk HPV types from DNA obtained from vaginal and cervical swab samples. Based on qPCR (real-time Polymerase Chain Reaction) technology, it allows testing with either extracted viral DNA or directly with samples inserted into Multinow solution—offering fast and accurate results.
Working Principle
The MultiPap+ HPV Genotyping Kit enables the amplification of viral DNA through qPCR to identify rare high-risk HPV genotypes. The test is structured into three reaction wells, each detecting different sets of HPV types:
- Well 1: HPV31, HPV33, HPV35, HPV59
- Well 2: HPV39, HPV68, HPV69
- Well 3: HPV51, HPV52, HPV56, HPV58
Direct sample processing is enabled by placing swabs into Multinow Sample Collection Tubes, bypassing the need for prior DNA isolation in many cases
Overview of MultiPap+ HPV Genotyping Kit
Target HPV Types | HPV31, HPV33, HPV35, HPV39, HPV51, HPV52, HPV56, HPV58, HPV59, HPV68, HPV69 |
Analysis Time | Approximately 1 hour |
Cycle Count | 40 cycles |
Sample Type | Vaginal and cervical swab samples |
Control Types | Negative and positive controls |
Storage Conditions | Store at -20°C; thaw at room temperature before use |
Operating Temperature | Thermal cycling program adapted to the qPCR instrument |
Compatibility | Compatible with standard qPCR devices |
Application Protocol
To ensure accurate test results with the MultiPap HPV Diagnostic Kit, follow the steps below:
- Collect vaginal or cervical swab samples using a sterile swab.
- Transfer the swab into a tube containing Multinow sample solution and mix thoroughly.
- DNA isolation is optional; the process can be performed directly from the Multinow solution.
- If isolation is required, follow the procedure specified in the protocol.
- Combine Master Mix and Primer Mixes 1, 2, and 3 in appropriate volumes as per manufacturer instructions.
- Add the mixture to designated wells on the PCR plate using a pipette.
- Include the positive and negative controls in each test group.
- Place the prepared plate into the qPCR device.
- Run the system using the specified thermal cycle program (40 cycles).
- Analyze results using the qPCR software.
- Interpret fluorescence signal data to determine if the result is Positive, Negative, or Invalid.
Result Interpretation
| Result Type | Description |
| Positive | Fluorescent signal detected due to amplification of target HPV gene regions |
| Negative | No amplification detected, indicating the test performed correctly |
| Invalid | If MultiPap HPV screening kit gives a positive result via primer mix-2 on the FAM channel but the genotyping kit gives a negative result, the test has failed and must be repeated |